Proteome-Wide Mapping of Protein Kinase Targets in Response to Stress
活动时间： 11月06日08时30分
地 点 ： 理科群3号楼A121室
讲座内容：
Protein kinases are majorregulatory components in almost all cellular processes in eukaryotic cells. Byadding phosphate groups, protein kinases regulate the activity, localization,protein-protein interactions, and other features of their target proteins. Itis known that protein kinases, such as SNF1-related protein kinases 2 and 3(SnRK2 and SnRK3), calcium dependent protein kinases (CDPKs), and mitogenactivated protein kinases (MAPKs) are central components in plant response toenvironmental stresses, such as drought, high salinity, cold, and pathogenattack. Although phosphorylation-dependent signaling plays critical roles inplant stress biology, only a small number of targets of these protein kinaseshave been identified, and consequently how these protein kinases regulatedownstream biological processes and mediate stress responses are still largelyunknown. In this study, we developed an isotope labeled protein kinaseassay-based approach with enriched phosphoproteomes as kinase-substrate poolsto identify the putative substrates of eight protein kinases that function inplant abiotic and biotic stress responses. As a result, we identified more thanfive thousand putative targets from 12 protein kinase-stimuli combinations. Wealso analyzed the conserved motif and functional ontology of these putativekinase targets. These results provide comprehensive information on the role ofthese protein kinases in controlling cellular activities and constitute avaluable resource for further studies on the mechanisms underlying plantresponse to environmental stresses.
主讲人介绍：
王鹏程，2010年河南大学获得植物学博士学位，2011-2017年在美国普渡大学从事博士后研究。2017年入选中组部第13批“青年千人计划”，在中国科学院分子植物卓越中心/上海植物逆境生物学研究中心担任研究员。主要从事植物蛋白激酶和非生物胁迫信号转导研究。综合利用遗传学、分子生物学和蛋白组学等研究技术解析一些重要蛋白激酶SnRK2、MAPK等及其底物在植物非生物逆境胁迫应答过程中的作用。在植物活性氧信号转导，磷酸化蛋白组学以及植物激素ABA信号转导途径方面取得一系列研究成果。代表性研究成果发表在MolecularCell、PlantCell、PNAS、PlantPhysiology等国际权威性SCI期刊上。
发布时间：2018-10-30 15:36:37